p p70s6  (Cell Signaling Technology Inc)

Journal: Biochemical and biophysical research communications

Article Title: Integration of metabolomics and transcriptomics to reveal metabolic characteristics and key targets associated with lncRNA Vof-16 in H19-7 cells.

doi: 10.1016/j.bbrc.2024.150855

Figure Lengend Snippet: Fig. 5. The effect of lncRNA Vof-16 on hippocampal neuron cells through autophagy and mTOR pathway. (A) Autophagy-labeled protein LC3 (red) was detected by immunofluorescence. Images were collected under the Laser confocal high-content imaging analysis system and its mean fluorescence intensity was quantified by Image J; n = 9, scale bars, 20 μm. (B–C) Autophagy-associated proteins LC3I/II, ATG3, ATG12 levels were measured by western blotting. Values represented mean ± SD. n = 3, *P < 0.05, ***P < 0.001 versus the Control group. (D–E) The downstream effector of mTOR signaling (phosphorylation of p70 S6 kinase) was quantitated by western blotting. Values were shown as mean ± SD. n = 3, *P < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Antibodies against LC3 A/B, ATG12, ATG3, p-p70S6 Kinase, and β-actin were purchased from CST (1:1000 dilution). β-Tubulin antibody was purchased from Affinity (1:20000 dilution).

Techniques: Labeling, Immunofluorescence, Imaging, Fluorescence, Western Blot, Control, Phospho-proteomics

Journal: Cancers

Article Title: Cytotoxic Autophagy: A Novel Treatment Paradigm against Breast Cancer Using Oleanolic Acid and Ursolic Acid

doi: 10.3390/cancers16193367

Figure Lengend Snippet: List of antibodies used in this study.

Article Snippet: p-P70S6 Kinase (Ser371) , Cell Signaling Technology 9208 , AB_330990.

Techniques: Fluorescence